rabbit anti btg2  (Proteintech)

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: Mutual DEGs between periodontitis and DKD ( a ) Venn diagram of mutual DEGs between periodontitis (GSE16134, GSE10334) and DKD from GEO (GSE96804) database. There were a total of 12 co-expressed DEGs screened. ( b ) PCA analysis of DKD. Clustered samples indicated little difference between the samples. ( c ) Volcano plot of DEGs. Log2 FC > 1 or <-1, an adjusted P-value < 0.05. ( d ) Histogram of DEGs. Compared to the control, BTG2, FPR1, FCGR3B, FOS, HBA2, SELL, PPBP were decreased, and C3, IGKV1-17, IGKV1-5, MMP7 and CCL18 were increased. ( e ) BTG2 expression in diabetic kidney and healthy living donor from Nephroseq V5 online nephropathy database. Compared to the normal, BTG2 expression in DKD was significantly decreased. ( f ) The correlation between BTG2 expression and proteinuria. With higher BTG2 expression level, DKD patients have less proteinuria. P < 0.05 has a statistical significance. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Control, Expressing

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: Pearson correlation analysis for BTG2 from DKD samples correlation R value stands for correlation coefficient, while positive values represent positive correlation and negative values represent negative correlation. The greater the absolute value of R, the stronger the correlation. Coefficient > 0.5 or < -0.5, P < 0.05

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques:

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: DEGs selected by BTG2 expression level ( a ) Comparison of BTG2 differential expression in DKD patients. Dividing DKD patients into BTG2 higher and lower expression groups, higher BTG2 group has significantly higher BTG2 expression level than the lower. ( b ) PCA of BTG2 differential expression in DKD dataset. Clustered samples indicated little difference between the samples. ( c ) Heatmap of DEGs. Demonstrated the synergistic relationship between DEGs and BTG2 expression. ( d ) Correlation heatmap between BTG2 and the coding genes. ( e ) Circos plot between BTG2 and the coding genes. Log2 FC > 1.5 or <-1.5, adjust P value < 0.05

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Comparison

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: DEGs functional analysis a: Bubble diagram based on gene sets h.all.v7.1.symbols.gmt . b. Bubble diagram based on gene sets c2.cp.kegg.v7.1.symbols.gmt . Count represents bubble size and color represents adjusted P . GeneRatio = count / number of annotated gene. c: GSEA of BTG2 enrichment pathway. Each bar represents a gene, and the vertical coordinates represented the enrichment scores of the genes. Positive scores are regarded as gene activation, and negative scores are regarded as gene inhibition. d: PPI network constructed by cytoscape software. Parameter setting: Degree Cutoff: 2, Node Score Cutoff: 0.2, K-Core: 2, Max.Depth: 100. Cluster 1: 4.571 points, Cluster 2: 4.25 points, cluster 3: 3.6 points

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Functional Assay, Activation Assay, Inhibition, Construct, Software

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: BTG2 optimal exposure time determination and expression change to HG a :the BTG2 mRNA expression level under different HG (30mmol/L) exposure time detected by aPCR. When exposed to HG for 24 h, BTG2 has the lowest expression level. b : BTG2 expression change when exposed to HG in immunofluorescence assay. *, P < 0.05; **, P < 0.01

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Immunofluorescence

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 and EMT and apoptotic related proteins a : Western blot between BTG2 and nephrin, α-SMA, vimentin, Bim. b : Histogram of BTG2’s relation with Bim. Compared to the control, exposed to HG had a higher Bim/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower Bim/β-actin ratio. c : Histogram of BTG2’s relation with α-SMA. Compared to the control, exposed to HG had a higher α-SMA/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower α-SMA/β-actin ratio. d : Histogram of BTG2’s relation with vimentin. Compared to the control, exposed to HG had a higher vimentin/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower vimentin/β-actin ratio. e: Histogram of BTG2’s relation with nephrin. Compared to the control, exposed to HG had a lower nephrin/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a higher α-SMA/β-actin ratio. Internal reference control: β-actin; NC: negative control; *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Western Blot, Control, Expressing, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 expression and podocytes migration ability a . Wound healing assay of BTG2 expression to cell migration ability. The wound closure rates were observed to compare cell migration ability under the condition of BTG2 underexpression and overexpression. b : Relative % of wound closure. Compared to the control, under-expressing BTG2 and exposed to HG had better wound closure. Compared to HG exposure, over-expressing BTG2 under HG had worse wound closure. NC: negative control; *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Migration, Wound Healing Assay, Over Expression, Control, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 expression and podocyte apoptosis a : Flow apoptosis assay of BTG2 to cell apoptosis. b : Apoptosis cells (%) with BTG2 expression change. Compared to the control, exposed to HG had much more apoptotic cells. Compared to HG exposure, over-expressing BTG2 under HG had less apoptotic cells. NC, negative control; si, under-express; oe, over-express. *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Apoptosis Assay, Control, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 and mTORC1 signaling pathway and autophagy related proteins a : Western blot of BTG2 to RPS6KB1 and mTORC1. Compared to the control, exposed to HG had higher p-RPS6KB1/RPS6KB1 and p-MTORC1/MTORC1 ratios. Compared to HG exposure, over-expressing BTG2 under HG had lower p-RPS6KB1/RPS6KB1 and p-MTORC1/MTORC1 ratios. b : Western blot of BTG2 to autophagy protein LC3. Compared to the control, exposed to HG had a lower LC3II/LC3I ratio. Compared to HG exposure, over-expressing BTG2 under HG had a higher LC3II/LC3I ratio. Internal reference control: β-actin; NC: negative control; *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Western Blot, Control, Expressing, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: Mutual DEGs between periodontitis and DKD ( a ) Venn diagram of mutual DEGs between periodontitis (GSE16134, GSE10334) and DKD from GEO (GSE96804) database. There were a total of 12 co-expressed DEGs screened. ( b ) PCA analysis of DKD. Clustered samples indicated little difference between the samples. ( c ) Volcano plot of DEGs. Log2 FC > 1 or <-1, an adjusted P-value < 0.05. ( d ) Histogram of DEGs. Compared to the control, BTG2, FPR1, FCGR3B, FOS, HBA2, SELL, PPBP were decreased, and C3, IGKV1-17, IGKV1-5, MMP7 and CCL18 were increased. ( e ) BTG2 expression in diabetic kidney and healthy living donor from Nephroseq V5 online nephropathy database. Compared to the normal, BTG2 expression in DKD was significantly decreased. ( f ) The correlation between BTG2 expression and proteinuria. With higher BTG2 expression level, DKD patients have less proteinuria. P < 0.05 has a statistical significance. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Control, Expressing

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: Pearson correlation analysis for BTG2 from DKD samples correlation R value stands for correlation coefficient, while positive values represent positive correlation and negative values represent negative correlation. The greater the absolute value of R, the stronger the correlation. Coefficient > 0.5 or < -0.5, P < 0.05

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques:

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: DEGs selected by BTG2 expression level ( a ) Comparison of BTG2 differential expression in DKD patients. Dividing DKD patients into BTG2 higher and lower expression groups, higher BTG2 group has significantly higher BTG2 expression level than the lower. ( b ) PCA of BTG2 differential expression in DKD dataset. Clustered samples indicated little difference between the samples. ( c ) Heatmap of DEGs. Demonstrated the synergistic relationship between DEGs and BTG2 expression. ( d ) Correlation heatmap between BTG2 and the coding genes. ( e ) Circos plot between BTG2 and the coding genes. Log2 FC > 1.5 or <-1.5, adjust P value < 0.05

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Comparison, Quantitative Proteomics

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: DEGs functional analysis a: Bubble diagram based on gene sets h.all.v7.1.symbols.gmt . b. Bubble diagram based on gene sets c2.cp.kegg.v7.1.symbols.gmt . Count represents bubble size and color represents adjusted P . GeneRatio = count / number of annotated gene. c: GSEA of BTG2 enrichment pathway. Each bar represents a gene, and the vertical coordinates represented the enrichment scores of the genes. Positive scores are regarded as gene activation, and negative scores are regarded as gene inhibition. d: PPI network constructed by cytoscape software. Parameter setting: Degree Cutoff: 2, Node Score Cutoff: 0.2, K-Core: 2, Max.Depth: 100. Cluster 1: 4.571 points, Cluster 2: 4.25 points, cluster 3: 3.6 points

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Functional Assay, Activation Assay, Inhibition, Construct, Software

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: BTG2 optimal exposure time determination and expression change to HG a :the BTG2 mRNA expression level under different HG (30mmol/L) exposure time detected by aPCR. When exposed to HG for 24 h, BTG2 has the lowest expression level. b : BTG2 expression change when exposed to HG in immunofluorescence assay. *, P < 0.05; **, P < 0.01

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Immunofluorescence

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 and EMT and apoptotic related proteins a : Western blot between BTG2 and nephrin, α-SMA, vimentin, Bim. b : Histogram of BTG2’s relation with Bim. Compared to the control, exposed to HG had a higher Bim/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower Bim/β-actin ratio. c : Histogram of BTG2’s relation with α-SMA. Compared to the control, exposed to HG had a higher α-SMA/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower α-SMA/β-actin ratio. d : Histogram of BTG2’s relation with vimentin. Compared to the control, exposed to HG had a higher vimentin/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a lower vimentin/β-actin ratio. e: Histogram of BTG2’s relation with nephrin. Compared to the control, exposed to HG had a lower nephrin/β-actin ratio. Compared to HG exposure, over-expressing BTG2 under HG had a higher α-SMA/β-actin ratio. Internal reference control: β-actin; NC: negative control; *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Western Blot, Control, Expressing, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 expression and podocytes migration ability a . Wound healing assay of BTG2 expression to cell migration ability. The wound closure rates were observed to compare cell migration ability under the condition of BTG2 underexpression and overexpression. b : Relative % of wound closure. Compared to the control, under-expressing BTG2 and exposed to HG had better wound closure. Compared to HG exposure, over-expressing BTG2 under HG had worse wound closure. NC: negative control; *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Migration, Wound Healing Assay, Over Expression, Control, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 expression and podocyte apoptosis a : Flow apoptosis assay of BTG2 to cell apoptosis. b : Apoptosis cells (%) with BTG2 expression change. Compared to the control, exposed to HG had much more apoptotic cells. Compared to HG exposure, over-expressing BTG2 under HG had less apoptotic cells. NC, negative control; si, under-express; oe, over-express. *, P < 0.05; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Expressing, Apoptosis Assay, Control, Negative Control

Journal: BMC Medical Genomics

Article Title: Deciphering the molecular nexus of BTG2 in periodontitis and diabetic kidney disease

doi: 10.1186/s12920-024-01915-6

Figure Lengend Snippet: The relationship between BTG2 and mTORC1 signaling pathway and autophagy related proteins a : Western blot of BTG2 to RPS6KB1 and mTORC1. Compared to the control, exposed to HG had higher p-RPS6KB1/RPS6KB1 and p-MTORC1/MTORC1 ratios. Compared to HG exposure, over-expressing BTG2 under HG had lower p-RPS6KB1/RPS6KB1 and p-MTORC1/MTORC1 ratios. b : Western blot of BTG2 to autophagy protein LC3. Compared to the control, exposed to HG had a lower LC3II/LC3I ratio. Compared to HG exposure, over-expressing BTG2 under HG had a higher LC3II/LC3I ratio. Internal reference control: β-actin; NC: negative control; *, P < 0.05; **, P < 0.01; ***, P < 0.001

Article Snippet: Rabbit anti- BTG2 antibody (bs-0031R) was purchased from Beijing Bioss Biotechnology Co., Ltd while α-SMA, vimentin, Bim, pMTOR, MTOR, RPS6KB1, LC3 antibodies and nephrin, pRPS6KB1 antibodies were purchased from Wuhan Proteintech Group, Inc and Jiangsu Affinity Biosciences Ltd, respectively.

Techniques: Western Blot, Control, Expressing, Negative Control

Journal: Oncology Research

Article Title: Betulinic acid-mediating miRNA-365 inhibited the progression of pancreatic cancer

doi: 10.32604/or.2023.026959

Figure Lengend Snippet: Sequences of target genes

Article Snippet: The antibodies used in the experiment were BTG2 (Abcam, AB244260), IL-6 (Abcam, AB233706), p-AKT (CST, 4060S), AKT (CST, 4685S), p-STAT3 (CST, 9145S) and STAT3 (CST, 9139S), respectively.

Techniques:

Journal: Oncology Research

Article Title: Betulinic acid-mediating miRNA-365 inhibited the progression of pancreatic cancer

doi: 10.32604/or.2023.026959

Figure Lengend Snippet: Expressions of miR365, IL-6, AKT and STAT3 were downregulated by BA in vivo (A) H&E pathological section of pancreatic cancer (200X); (B) The level of miR365 was reduced by BA; (C) The relationship between miR-365 and BTG2 was analyzed using the Targetscan database; (D) Determination of mRNA level of BTG2 in tumor tissue; (E) BA inhibited the protein level of IL-6; (F) Expression of AKT were determined by immunohistochemistry (400X); (G) Expression of STAT3 were determined by immunohistochemistry (400X). * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The antibodies used in the experiment were BTG2 (Abcam, AB244260), IL-6 (Abcam, AB233706), p-AKT (CST, 4060S), AKT (CST, 4685S), p-STAT3 (CST, 9145S) and STAT3 (CST, 9139S), respectively.

Techniques: In Vivo, Expressing, Immunohistochemistry

Journal: Oncology Research

Article Title: Betulinic acid-mediating miRNA-365 inhibited the progression of pancreatic cancer

doi: 10.32604/or.2023.026959

Figure Lengend Snippet: BA inhibits miR-365 expression, causing changes in BTG2/BTG2 and thus inhibiting IL-6/AKT/STAT3 expression (A) The expression of BTG2/IL-6 is measured using QT-PCR in two cell strains treated with BA and miR-365 inhibitors; (B) The expression of AKT/STAT3 is measured using QT-PCR in two cell strains treated with BA and miR-365 inhibitors; (C) WB quantified the changes in BTG2/IL-6/STAT3/P-STAT3/AKT/P-AKT expression in two pancreatic cancer cells treated with BA and miR-365 inhibitor. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: The antibodies used in the experiment were BTG2 (Abcam, AB244260), IL-6 (Abcam, AB233706), p-AKT (CST, 4060S), AKT (CST, 4685S), p-STAT3 (CST, 9145S) and STAT3 (CST, 9139S), respectively.

Techniques: Expressing